Technical Data
C5838-02F
Claudin 1 (Loop1) (Claudin-1, Senescence-associated Epithelial Membrane Protein, CLD1, SEMP1, CLDN1, UNQ481/PRO944)
Description:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. CLDN1, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Loop1 region of human CLDN1 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CLDN1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kinugasa,T., Anticancer Res. 27(6A), 3729-3734 (2007). 2.Paschoud,S., Mod. Pathol. 20(9), 947-954 (2007). 3.Morohashi,S., Int. J. Mol. Med. 20(2), 139-143 (2007). 4.Krajewska,M., Prostate 67(9), 907-910 (2007).