Technical Data
C5838-02G
Claudin 1 (Loop2) (Claudin-1, Senescence-associated Epithelial Membrane Protein, CLD1, SEMP1, CLDN1, UNQ481/PRO944)
Description:
CLDN1, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Loop2 region of human CLDN1 (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CLDN1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kinugasa,T., Anticancer Res. 27(6A), 3729-3734 (2007). 2.Paschoud,S., Mod. Pathol. 20(9), 947-954 (2007). 3.Morohashi,S., Int. J. Mol. Med. 20(2), 139-143 (2007). 4.Krajewska,M., Prostate 67(9), 907-910 (2007).