Technical Data
Claudin 1 (Claudin-1, CLD1, CLDN1, ILVASC, Senescence-associated Epithelial Membrane Protein, SEMP1, UNQ481/PRO944)
Claudin1, a 22kD integral membrane protein, is a member of Claudin family. It is a critical component of tight junction strands and consists of 2 extracellullar loops, 4 hydrophobic transmembrane domains and a cytoplasmic tail containing PDZ-binding motif. Studies on MDCK cells and knock-out mice have revealed that Claudin-1 plays a vital role in forming tight junctions that serve as a physical barrier preventing free passage of solutes and water through the paracellular spaces. Its role has also been suggested in beta-catenin-Tcf/LEF signaling pathway. Mutations in Claudin-1 have been strongly suggested in NISCH syndrome. Northern-Blot analysis detected a ubiquitous expression of Claudin-1 with highest in liver and kidney.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Breast carcinoma, skin

Cellular Localization:
Cytoplasmic and cell membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to C-terminal of human Claudin 1.
Supplied as a liquid in 0.05% sodium azide.
Recognizes human Claudin 1. Species Crossreactivity: mouse, canine, rat, and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miwa Oncol Res 12 (11-12) 469-476 (2000). 2. Balkovetz Am J Physiol Renal Physiol 290; F572-F579 (2006)