Technical Data
C5838-02J
Claudin 1 (Claudin-1, CLD1, CLDN1, ILVASC, Senescence-associated Epithelial Membrane Protein, SEMP1, UNQ481/PRO944)
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Technical Data |
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C5838-02J |
Claudin 1 (Claudin-1, CLD1, CLDN1, ILVASC, Senescence-associated Epithelial Membrane Protein, SEMP1, UNQ481/PRO944) |
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Description: Claudin1, a 22kD integral membrane protein, is a member of Claudin family. It is a critical component of tight junction strands and consists of 2 extracellullar loops, 4 hydrophobic transmembrane domains and a cytoplasmic tail containing PDZ-binding motif. Studies on MDCK cells and knock-out mice have revealed that Claudin-1 plays a vital role in forming tight junctions that serve as a physical barrier preventing free passage of solutes and water through the paracellular spaces. Its role has also been suggested in beta-catenin-Tcf/LEF signaling pathway. Mutations in Claudin-1 have been strongly suggested in NISCH syndrome. Northern-Blot analysis detected a ubiquitous expression of Claudin-1 with highest in liver and kidney. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:100-1:200 Optimal dilutions to be determined by the researcher. Positive Control: Breast carcinoma, skin Cellular Localization: Cytoplasmic and cell membrane Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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