Technical Data
C5838-16
Claudin 2 (CLDN2)
Description:
Tight Junctions (TJ) are specialized membrane domains that seal the intercellular spaces in epithelia and endothelia and thus contribute to the permeability barrier between lumenal and interstitial compartments. Tight junctions not only separate distinct physiological compartments, but they also confer selectivity to the transepithelial ux of molecules and ions through the intercellular spaces between the epithelial cells, the so-called paracellular pathway. In addition, tight junctions limit the diffusion of lipids between the apical and basolateral plasma membrane domain. Claudin and Occludin, the two types of four-transmembrane domain proteins, are the major integral membrane components of the mammalian tight junction. So far, 20 distinct claudins have been identi ed. Mouse Claudin-2 was discovered in 1998 as a 230aa integral membrane protein localizing at tight junctions with an approximate molecular weight of 22.9kD. Claudin-1 and Claudin-2 are structurally related, and are in fact 38% identical at the amino acid sequence level. The expression of Claudin-2 is restricted to the liver and kidney, with small amounts found in the brain.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (Rat): 1-3ug/ml
Western Blot (Human, mouse, rat, canine): 1-3ug/ml; Non-specific band at ~27kD observed in some lysates. In Caco-2 cells, this band appears stronger than the correct band at 22kD.
Immunhistochemistry (FFPE): 1-2ug/ml; Confirmed with human normal colon, spleen and thyroid, colon and breast cancer tissues. Heat induced epitope retrieval with EDTA buffer, pH 8, is required prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b3G95Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of the mouse Claudin-2 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the mouse Claudin-2 protein at ~23kD. Reacts with liver and kidney homogenates. Species Crossreactivity: human (Caco-2 cell lysates), rat, canine (MDCK cell lysates)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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