Technical Data
C5838-17A
Claudin 2 (Claudin-2, CLDN2, PSEC0059, SP82, UNQ705/PRO1356)
Description:
Claudin-2, a 22kD integral membrane protein, is a member of Claudin family and was discovered along with Claudin-1. It is also a vital component of tight junction strands and consists of 2 extracellullar loops, 4 hydrophobic transmembrane domains and a cytoplasmic tail containing PDZ-binding motif. Studies on MDCK cells suggest that Claudin-2 determines the overall transepithelial membrane resistance properties and increases conductance by forming cation-selective channels in tight junction of epithelial cells. Claudin-2 is differentially expressed in the intestine and has been suggested to be involved in the regulation of intestinal cell monolayer barrier function through immune modulators such as interleukins.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Ovarian carcinoma, tonsil

Cellular Localization:
Cytoplasmic and cell membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to C-terminal of human Claudin 2.
Purity:
Serum
Form
Supplied as a liquid in 0.05% sodium azide.
Specificity:
Recognizes human Claudin 2. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amasheh et.al J Cell Sci 115; 4969-4976 (2002). 2. Escaffit et.al J Cell Physiol 203 (1) 15-26 (August 27, 2004).