Technical Data
Claudin 2 (Claudin-2, CLDN2, PSEC0059, SP82, UNQ705/PRO1356)
Claudin-2, a 22kD integral membrane protein, is a member of Claudin family and was discovered along with Claudin-1. It is also a vital component of tight junction strands and consists of 2 extracellullar loops, 4 hydrophobic transmembrane domains and a cytoplasmic tail containing PDZ-binding motif. Studies on MDCK cells suggest that Claudin-2 determines the overall transepithelial membrane resistance properties and increases conductance by forming cation-selective channels in tight junction of epithelial cells. Claudin-2 is differentially expressed in the intestine and has been suggested to be involved in the regulation of intestinal cell monolayer barrier function through immune modulators such as interleukins.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:100-1:200
Optimal dilutions to be determined by the researcher.

Positive Control:
Ovarian carcinoma, tonsil

Cellular Localization:
Cytoplasmic and cell membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to C-terminal of human Claudin 2.
Supplied as a liquid in 0.05% sodium azide.
Recognizes human Claudin 2. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amasheh J Cell Sci 115; 4969-4976 (2002). 2. Escaffit J Cell Physiol 203 (1) 15-26 (August 27, 2004).