Technical Data
C5838-18
Claudin 2 (CLDN2)
Description:
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:100 30 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive control:
Normal colon and colon carcinoma.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide derived from the C-terminus of mouse Claudin 2. Cellular Localization: Cell Membrane. MW of Antigen: 22-24kD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 5mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes mouse Claudin 2. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.