Technical Data
C5838-25
Claudin 3 (CLDN3)
Description:
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. The 20 known claudin proteins are major structural components of tight junctions.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1.0ug/ml
Western Blot: 0.5-3ug/ml
Immunohistochemistry: 0.1-1.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
A synthetic peptide from the C-terminus of mouse Claudin-3
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes mouse Claudin-3 at 22kD. Species Crossreactivity: Mouse liver and lung tissue extract and canine MDCK and human MCF-7 cell lysates by Western Blot and formalin-fixed, paraffin-embeddedd human normal colon and colon cancer tissues by Immunohistochemistry.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.