		Technical Data
C5838-25C	Claudin 3 (Claudin-3, CLDN3, C7orf1, Clostridium perfringens Enterotoxin Receptor 2, CPE-receptor 2, CPETR2, CPE-R 2, CPE-R2, CPETR2, Rat Ventral Prostate.1 Protein Homolog, RVP1, hRVP1)

Description:
Claudins are integral membrane proteins involved in the assembly and function of tight junction strands. Claudins have 4 transmembrane domains and 2 extracellular loops and C-terminal tail containing PDZ-binding motifs and YV sequences. Claudin 3, a human homolog of rat RVP1, is a unique member of this family. It is a low-affinity receptor for Clostridium perfringes entertoxin capable of mediating toxin-dependent cytolysis. Claudin-3 has been found to be phosphorylated and up-regulated in various cancer types suggesting a potential role in tumorigenesis.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Small intestine

Cellular Localization:
Cytoplasmic and cell membrane

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to C-terminal of human Claudin 3.

Purity:

Serum

Form

Supplied as a liquid in 0.05% sodium azide.

Specificity:

Recognizes human Claudin 3. Species Crossreactivity: rat and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Turksen et.al J Cell Sci 117; 2435-2447 (2004). 2. Agarwal et.al Cancer Res 65; 7378-7385 (August 15, 2005).