Technical Data
C5838-26
Claudin 3 (CLDN3)
Description:
Tight junctions (TJ) are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a sort of “molecular gasket” that prevents ions, water and other molecules from leaking between cells. Claudin-3 is expressed in junctions of the duct epithelia and acinar cells as well as uniformly expressed from the periportal to the pericentral hepatocytes in the liver. Also, Claudin-3 was found to be present or secreted by many of the genes up-regulated in ovarian cancer.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: (paraffin-embedded sections). Antigen retrieval by boiling in 10mM sodium citrate (pH 6.0) is needed.
Optimal dilutions to be determined by the researcher.

Posittive Control:
Colon.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide from the C-terminus of mouse Claudin 3.
Purity:
Purified.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes mouse Claudin 3. Species Crossreactivity: human and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hough C et al. Large-Scale Serial Analysis of Gene Expression Reveals Genes Differentially Expressed in Ovarian Cancer. Cancer Research 60, 6281- 6287 (2000). 2. Long H et al. Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium. Cancer Research 61(21), 7878-7881 (2001). 3. Shi ZR, et al. A comparison of three immunoperoxidase techniques for antigen detection in colorectal carcinoma tissues. J Histochem Cytochem 36(3):317-322, (1988). 4. Kiernan JA. Histological and Histochemical Methods: Theory and Practice . New York: Pergamon Press, (1981). 5. Sheehan DC and Hrapchak BB. Theory and Practice of Histotechnology . St. Louis: C.V. Mosby Co., (1980).