Technical Data
C5838-35
Claudin 4 (CLDN4)
Description:
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. The 20 known claudin proteins are major structural components of tight junctions. Claudin 4 is overexpressed in pancreatic cancer tissues and cell lines. Expression has been localized at junctions of the duct epithelia and acinar cells in rat pancreas. Overexpression of claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in sodium permeability. Membrane staining is usually seen in most tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): Requires Citrate Buffer pH 6.0 Heat Induced Epitode Retrival (HIER).
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Colon Tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13G97Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal of human Claudin 4.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human Claudin 4. Expected Staining Pattern: Membrane.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rahner, C., et al., Gastroenterology 120(2): 411-422 (2001). 2. Van Itallie, C., et al., J. Clin. Invest. 107(10): 1319-1327 (2001).