Technical Data
C5838-35B
Claudin 4 (CLDN4)
Description:
Claudin-4 belongs to the 20-member family of claudins, transmembrane proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
ELISA (Immunogen): 0.1-1ug/ml
Immunoprecipitation (mouse): 1-3ug/ml
Western Blot (mouse, rat): 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of the mouse Claudin-4 protein.
Purity:
Purified by epitope affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Claudin-4. On Western blots a 22kD band is observed and a 44kD band representing dimerized protein may be observed. Reactivity has been confirmed with mouse and rat kidney homogenates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morita K, et al. J Cell Biol 147:185-194, 1999. 2. Van Itallie CM, et al. J Clin Invest 107(10):1319-1327, 2001. 3. Hough CD, et al. Cancer Res 60:6281-6287, 2001. 4. Rangel LB, et al. Clin Cancer Res 9:2567-2575, 2003. 5. Michl P, et al. Gastroenterology 121(3):678-684, 2001.