		Technical Data
C5838-36	Claudin 4 (CLDN4)

Description:
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:25 for 30 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by researcher.

Positive Control: Tonsil and Ovarian carcinoma.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

A synthetic peptide derived from the C-terminus of human Claudin 4. Cellular Localization: Cell membrane and cytoplasmic. MW of Antigen: 22-24kD

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes human Claudin 4. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.