Technical Data
C5838-61
Claudin 12 (CLDN12)
Description:
Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton mediated by ZO-1. Claudin 12 expression has been observed in the lung, brain, kidney, skin, urinary bladder and ear.

Applications:
Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunofluorescence: 5ug/ml, performed using ethanol-fixed mouse colon.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse lung, colon, and kidney homogenates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12. Species Sequence Homology: human
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Claudin 12 protein at ~27kD. Crossreactivity has been confirmed on mouse lung, colon and kidney homogenates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sawada, N., et al., Med. Electron Microsc. 36(3): 147-56 (2003). 2. Strausberg, R.L., et al., PNAS 99(26): 16,899-16,903 (2002). 3. Kiuchi-Saishin, Y., et al., J. Am. Soc. Nephrol. 13(4): 875-886 (2002). 4. Brandner, J.M., et al., Eur. J. Cell Biol. 81(5): 253-263 (2002). 5. Acharya, P., et al., Am. J. Physiol. Renal Physiol. 287(2): F305-F318 (2004). 6. Kitajiri, S.I., et al., Hear. Res. 187(1-2): 25-34 (2004).