Technical Data
C5838-61A
Claudin 12 (CLDN12)
Description:
Claudin-12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-12 is expressed on intestinal, bladder, and blood-brain barrier epithelial cells. Human Claudin-12 shares 92% aa sequence identity with mouse and rat Claudin-12.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug/ml labels 1x10e6 cells. Validated for use with CaCo2 human colorectal adenocarcinoma cell line.
Immunocytochemistry: 8-25ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a7H195Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa1-244 from human Claudin 12, expressed in HEK293 human embryonic kidney cell line (NP_036261).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Claudin 12. Stains human Claudin 12 transfectants but not irrelevant transfectants. Species Sequence Homology: mouse and rat: 92%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.