Technical Data
Claudin 14 (CLDN14, claudin 14, DFNB29)
Alternate Name: CLDN14, claudin 14, DFNB29

Domain Information:

Gene Ontology Terms: protein complex assembly

Peptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 23kD band observed in Human Liver lysate (calculated MW of 25.7kD according to NP_652763). Recommended concentration: 1-3ug/ml.

1ug/ml staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See C5838-75A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PabIgGAffinity Purified
100ug-20°CBlue IceHumanGoat
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes Human, Mouse, Rat Claudin 14
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wilcox ER, Burton QL, Naz S, Riazuddin S, Smith TN, Ploplis B, Belyantseva I, Ben-Yosef T, Liburd NA, Morell RJ, Kachar B, Wu DK, Griffith AJ, Riazuddin S, Friedman TB., Mutations in the gene encoding tight junction claudin-14 cause autosomal

recessive deafness DFNB29., Cell. 2001 Jan 12;104(1):165-72.