		Technical Data
C5838-75C	CLDN14 (Claudin-14, UNQ777/PRO1571)

Description:
These proteins consist of 4 hydrophobic transmembrane domains, 2 extracellular loops and short cytoplasmic tails at N and C-termini containing YV and PDZ-domain binding motifs. Claudin 14, a 25kD glycoprotein, is a novel member of this family. It is a vital component of inner ear tight junction strands. Its expression has been observed in sensory epithelium of inner organ of Corti, vestibular system, kidney and Liver. Mutations in Claudin 14 cause a congenital autosomal recessive form of nonsyndromic sensorineural deafness in humans.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
ELISA: >1:128,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Duodenal lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to SATHSGYRLNDYV, from the C-terminus of the protein sequence according to NP_036262; NP_652763.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human Claudin 14. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wilcox ER, Burton QL, Naz S, Riazuddin S, Smith TN, Ploplis B, Belyantseva I, Ben-Yosef T, Liburd NA, Morell RJ, Kachar B, Wu DK, Griffith AJ, Riazuddin S, Friedman TB. Mutations in the gene encoding tight junction claudin-14 cause autosomalrecessive deafness DFNB29. Cell. 2001 Jan 12;104(1):165-72. PMID: 11163249. 2. Ben-Yosef et.al Hum. Molec. Genet. 12: 2049-2061 (2003). 3. Turksen K et.al Journal of Cell Science 117, 2435-2447 (2004). 4. Christina.M.Van Itallie et.al Journal of Cell Science 118, 1427-1436 (2005).