Technical Data
C5838-85
Claudin 15 (CLDN15)
Description:
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called “barrier” function, the “fence” function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Claudin proteins have been shown to be major integral membrane proteins localized at tight junctions. Furthermore, tight junctions in different tissues appear to be composed of different claudin proteins.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 15-20ug/ml
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry (Paraffin): 10ug/ml (for paraffin-embedded sections HIER step needed, 5 minutes in 1mM EDTA, pH 8.0.) Staining was con rmed using human colon tissue.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13G99Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide corresponding to a sequence derived from the C-terminus of human Claudin-15.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human Claudin-15 protein at ~22kD. Reactivity is con rmed using MDCK cells transfected with human Claudin-15. Crossreactivity with rat Claudin-15 has not been observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morita K, et al., Proc Natl Acad Sci USA 96:511-516 (1999).