Technical Data
C5838-86
Claudin 15 (CLDN15)
Description:
Tight junctions create circumferential seals around cells in epithelial tissues that are critical for maintaining cell-cell adhesion. The “barrier” function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces, while the “fence” function maintains cell polarity. Tight junctions thus function as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton by ZO-1.
Claudin-15 is expressed in endothelial cells in the mouse kidney. Claudin-15 creates Na+ -selective paracellular channels, and charge-reversing mutations on the first extracellular domain of Claudin-15 create Cl- -selective paracellular channels. Claudin-15 has a high level of expression in the mouse small and large intestine.

Applicaitons:
Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen) (mouse): 14.5ug/ml
Immunofluorescence (mouse): 14.5ug/ml
Western Blot (mouse, human, rat): 1-3ug/ml detects a single band at ~20kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of mouse Claudin-15.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Claudin-15. Reactivity has been confirmed with mouse intestine (ileum and colon) homogenates, rat ileum homogenates, and human claudin-15-transfected Xenopus oocyte homogenates. Low levels of expression have also been observed in mouse kidney homogenates by Western Blot. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sawada N, et al. Med Electron Microsc 36(3):147-156, 2003. 2. Kiuchi-Saishin Y, et al. J Am Soc Nephrol 13:875-886, 2002. 3. Colegio OR, et al. Am J Physiol Cell Physiol 283:C142-7, 2002.