Technical Data
Claudin 16 (Paracellin 1, CLDN16)
Claudin 16, also known as Paracellin-1, is a renal tight junction protein required for paracellular magnesium resorption. It is localized in the intercellular junctions of the epithelial cells of the thick ascending limb of Henle’s loop (TAL), the distal tubule, and the collecting duct(6). Antibodies against Tamm-Horsfall protein (THP) (a TAL marker) are used to localize this specific structure of nephrons in kidney sections(1). Mg2+ resorption occurs in TAL(2), and Claudin 16 is required for this paracellular function(3). Failure in renal ‘handling’ of magnesium can result in hypermagnesemia, whose major direct toxicity implications are cardiovascular as well as renal failure. Recent studies have shown that the lack of Claudin-16 proteins contributes to the dysfunction of paracellular renal transport systems(5). Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called “barrier” function, the “fence” function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Claudin proteins have been shown to be major integral membrane proteins localized at tight junctions.
Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for formalin paraffin tissues. Other applications not tested.

Recommended Dilution:
Immuno uorescence: 1-3ug/ml
Immunohistochemistry (Frozen): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4°C (-20°C Glycerol)Blue IceRabbit
Synthetic peptide corresponding to the C-terminal region of human Claudin 16.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.01% sodium azide, before the addition of glycerol to 40.
Recognizes mouse Claudin 16. Immunostaining of frozen mouse kidney sections localizes Claudin 16 to the thick ascending limb (TAL) of Henle’s loop. This region was identi ed by an antibody against the Tamm-Horsfall protein, which is also localized to the TAL of Henle’s loop in the kidney.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bachmann S et al., 1990, Histochemistry 94:517-523. 2. Kelepouris E & Agus ZS, Sem. 1998, Nephrol. 18:58-73. 3. Simon DB, et al., Science , 285:103-106. 4. Morita K, et al., 1999, Proc Natl Acad Sci USA 96:511-516. 5. Hirano T, et al., 2000, Genome Research 10 (5):659-663. 6. Weber S, et al., 2001, J. Am. Soc. Nephrol. 12 (12):2664-2672.