Technical Data
C5838-91
Claudin 18 (CLDN18)
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Technical Data |
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C5838-91 |
Claudin 18 (CLDN18) |
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Description: Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach.1 Claudin-18 expression has also been reported in the inner ear.2 Applications: Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (frozen): 5ug/ml Immunofluorescence: 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse lung and stomach homogenates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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