Technical Data
C5839-65
CLEC4D (C-type Lectin Domain Family 4 Member D, CLCSF8, CLEC6, MCL)
Description:
CLEC4D (C-type lectin domain family 4 member D), also known as CLCSF8, CLEC-6, and MCL, is a 30kD type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215 amino acid (aa) protein that contains a 17 aa N-terminal cytoplasmic domain, a 21 aa TM segment, and a 177 aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118 aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Applications:
Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Flow Cytometry: 25ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b7H219Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
NS0-derived recombinant human CLEC4D (aa52-215) Accession # Q8WXI8
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human CLEC4D.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.