Technical Data
C5839-65A
CLEC4D (C-type Lectin Domain Family 4, Member D, C-type Lectin Superfamily Member 8, C-type Lectin-like Receptor 6, CLEC-6, CLECSF8, MCL)
Description:
CLEC4D (C-type lectin domain family 4 member D), also known as CLCSF8, CLEC-6, and MCL, is a 30kD type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215aa protein that contains a 17aa N-terminal cytoplasmic domain, a 21aa TM segment, and a 177aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human CLEC4D.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2B10K53Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
100 Tests/ml
Immunogen:
Purified, NS0-derived, recombinant human CLEC4D (aa52-215; Accession #Q8WXI8).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
Specificity:
Recognizes human CLEC4D. Species sequence homology: mouse (63%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.