Technical Data
C5839-65B
CLEC4D (C-type Lectin Domain Family 4, Member D, C-type Lectin Superfamily Member 8, C-type Lectin-like Receptor 6, CLEC-6, CLECSF8, MCL) (PE)
Description:
CLEC4D (C-type lectin domain family 4 member D), also known as CLCSF8, CLEC-6, and MCL, is a 30kD type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215aa protein that contains a 17aa N-terminal cytoplasmic domain, a 21aa TM segment, and a 177aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4D is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface.

Applications:
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul/10e6 cells, human periferal blood monocytes
Optimal dilutions to be determined by the researcher.

Hybridoma:
Myeloma with B cells from Balb/c mice.

Storage and Stability:
Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b11C7Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.02mg/ml
Immunogen:
Purified, NS0-derived, recombinant human CLEC4D (aa52-215; Accession # Q8WXI8).
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in sodium chloride, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human CLEC4D. Does not cross react with rhCLECSF9, rhCLECSF13, rhOCIL or rhOCILrp2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.