Technical Data
C5839-66
CLEC4E (C-type lectin domain family 4, member E)
Description:
CLEC4E is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma-chain (FcR-gamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50. Incubated for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence (IF-IC): 1:500
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse F0 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k10K398Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa41-219 from human CLEC4E expressed in E. coli (NP_055173).
Purity:
Purified by Protein G affinity chromatography. ( 95% SDS-PAGE)
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human CLEC4E.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishikawa E, et al. (2009) J Exp Med, 206(13):2879-88. 2. Matsunaga I, et al. (2009) J Exp Med, 206(13):2865-8. 3. Graham LM, et al. (2009) Cytokine, 48(1-2):148-55.