		Technical Data
C5839-72	CLEC9a (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046)

Description:
CLEC9a also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature human CLEC9a consists of a 35aa cytoplasmic domain with an ITAM-like motif, a 21aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of ~45-50kD N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2.5ug labels 10e6 cells
Optimal dilutions to be determined by the researcher.

Endotoxin: ~0.10EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	12K62	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml after reconstitution

Immunogen:

Recombinant corresponding to human CLEC9a expressed in NS0 cell line.

Purity:

Purified by Protein A/G affinity chromatography.

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

Specificity:

Recognizes human CLEC9A. No reactivity with recombinant human CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121. 2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33. 3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693. 4. Caminschi, I. et al. (2008) Blood 112:3264. 5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098. 6. Dudziak D. et. al.1(2007) Science 315:107. 7. Sancho, D. et al. (2009) Nature 458:899.