Technical Data
C5839-72
CLEC9a (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046)
Description:
CLEC9a also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature human CLEC9a consists of a 35aa cytoplasmic domain with an ITAM-like motif, a 21aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of ~45-50kD N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2.5ug labels 10e6 cells
Optimal dilutions to be determined by the researcher.

Endotoxin: ~0.10EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG112K62Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml after reconstitution
Immunogen:
Recombinant corresponding to human CLEC9a expressed in NS0 cell line.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human CLEC9A. No reactivity with recombinant human CLEC1, 2, 2A, 3B, 10A, 12B, 14A, rhCD302/CLEC13a, rhMICL, or recombinant mouse CLEC9a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121. 2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33. 3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693. 4. Caminschi, I. et al. (2008) Blood 112:3264. 5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098. 6. Dudziak D. et. al.1(2007) Science 315:107. 7. Sancho, D. et al. (2009) Nature 458:899.