Technical Data
C5839-72B
CLEC9a (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046) (APC)
Description:
CLEC9a also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature human CLEC9a consists of a 35aa cytoplasmic domain with an ITAM-like motif, a 21aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of ~45-50kD N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa57-264 of the extracellular domain, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Excitation: 620-650nm

Emission: 660-670nm

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
TypeIsotypeCloneGrade
MabIgG2A12K64Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceMouseRat
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa57-264 from mouse CLEC9a expressed in CHO-S cell line.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
Specificity:
Recognizes mouse CLEC9a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.