Technical Data
C5839-75
CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)
Description:
CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels up to 1x10e6 cells.
Optimal dilutions to be determined by the researcher.

Positive Control
Whole blood monocytes.

Flow Cytometry Protocol:
1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C5839-75 to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Freezing APC conjugates will result in a substantial loss of activity. APC is light sensitive.
TypeIsotypeCloneGrade
MabIgG2b11C8Affinity Purified
SizeStorageShippingSourceHost
100 tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.01mg/ml
Immunogen:
Recombinant protein corresponding to the extracellular domain of human CLEC5A expressed in NSO cells (Q9NY25).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity:
Recognizes human CLEC5A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.