Technical Data
C5839-75
CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)
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Technical Data |
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C5839-75 |
CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC) |
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Description: CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using whole blood monocytes. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of C5839-75 to 1-2.5x10e5 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light. |
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