Technical Data
Media
CLED Agar (Powder)
C5840
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Pancreatic Digest of Gelatin4.0
Beef Extract3.0
Casein Digest Peptone4.0
Lactose10.0
L-Cystine0.128
Bromothymol Blue0.02
Agar15.0
Total:36.1g/L
CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sandys. This medium supports the growth of urinary pathogens and gives good colonial differentiation of these bacteria. Lactose is the carbon source. Bromthymol blue is the indicator dye. bromthymol blue, Organisms that are lactose fermenting produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Since there are no salts
(electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. CLED Agar is recommended as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.


Appearance:
Light blue-green, homogeneous, free flowing powder

Solubility:
Light greyish-green, clear, complete

pH (3.6% 25C):
7.30.2

Microbiological Analysis:
Escherichia coli ATCC 25922: Growth Yellow Colonies
Proteus mirabilis ATCC 12453: Growth Clear Colonies
Salmonella typhimurium ATCC 14028 : Growth Clear Colonies
Staphylococcus aureus ATCC 25923: Growth Yellow Colonies

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions per Litler: Mix 36.1 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0C for 15 minutes.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.