Technical Data
C5840-93
CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)
Description:
The 63kD cytoskeleton-linking membrane protein (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation: 2ul
Immunocytochemistry: 1:1000
Immunohistochemistry: Frozen sections
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6D315Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human CLIMP63 from Caco-2 cells golgi membrane fraction.
Purity:
Purified by Protein G affinity chromatography and gel filtrated (FPLC), 95%.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
Specificity:
Recognizes human CLIMP63. Species Crossreactivity: monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schweizer, A., et al., J. Cell Sci. 104: 671 (1993). 2. Schweizer, A., et al., J. Cell Sci. 104: 685 (1993). 3. Schweizer, A., et al., J. Cell Biol. 126: 25 (1994). 4. Schweizer, A., et al., J. Cell Sci. 108: 2477 (1995). 5. Klopfenstein, D.R., et al., EMBO J. 17: 6168 (1998). 6. Klopfenstein, D.R., et al., J. Cell Biol. 153: 1287 (2001). 7. Breuza, L., et al., J. Biol. Chem. 279: 47,242 (2004).