Technical Data
CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)
The 63kD cytoskeleton-linking membrane protein (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.

Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation: 2ul
Immunocytochemistry: 1:1000
Immunohistochemistry: Frozen sections
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2a6D315Affinity Purified
100ug-20CBlue IceHumanMouse
~1mg/ml (after reconstitution)
Human CLIMP63 from Caco-2 cells golgi membrane fraction.
Purified by Protein G affinity chromatography and gel filtrated (FPLC) (> 95%).
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
Recognizes human CLIMP63. Species Crossreactivity: monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schweizer, A., et al., J. Cell Sci. 104: 671 (1993). 2. Schweizer, A., et al., J. Cell Sci. 104: 685 (1993). 3. Schweizer, A., et al., J. Cell Biol. 126: 25 (1994). 4. Schweizer, A., et al., J. Cell Sci. 108: 2477 (1995). 5. Klopfenstein, D.R., et al., EMBO J. 17: 6168 (1998). 6. Klopfenstein, D.R., et al., J. Cell Biol. 153: 1287 (2001). 7. Breuza, L., et al., J. Biol. Chem. 279: 47,242 (2004).