Technical Data
C5843-01
Clock
Description:
CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide per well.

Control Peptide:
C5843-40: Clock, Rabbit (dClock) Control Peptide

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceDrosophilaRabbit
Concentration:
~1mg/ml
Immunogen:
A 17aa C-terminus peptide of Drosophila Clock. (KLH coupled)
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes Drosophila Clock. The immunogen shows no significant sequence homology with mammalian Clock or Per proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bae, K. et al (1998) Mol. Cell. Biol. 18:61426151; 2. Allada, R. et al., (1998) Cell 93:791804; 3. Darli, T.K.. et al., (1998) Science 280:15991603.