Technical Data
C5843-02
Clock
Description:
CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

Application(s): Western blot: 110ug/ml using ECL. Immunohistochemistry: not tested. It is recommended that the antibody be tried at 220ug/ml. ELISA: 1:10,0001:100,000 using 50100ng of control peptide per well.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
A 20 amino acid peptide near the C-terminus of human Clock (1).
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human Clock. The immunogen shows no significant sequence homology with dPER or other known proteins. Species Reactivity: Human, the immungen sequence is 90% homologous with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Antoch, M.P. et al (1997) Cell 89:655667; King, D.P. et al., (1997) Cell 89:641653.