		Technical Data
C5843-02A	Clock

Description:
Clock is a basic helix-loop helix (bHLH) transcription factor that regulates circadian rhythms and sleep cycles. Clock contain a DNA-binding domain, a protein dimerization domain that heterodimerizes with BMAL1 and a glutamine-rich C terminus region. Clock dimerization with BMAL helps regulate nuclear translocation and binding of Clock to the E box elements stimulates circadian genes transcription.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-4 ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide: C5843-02A1

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide surrrounding aa842 of mouse Clock.

Purity:

Purified by immunoaffinitiy chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

Specificity:

Recognizes the 100kD and the ~62kD isoforms of mouse CLOCK. Species Crossreactivity: human, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.