Technical Data
C5843-16
Clock
Description:
Clock is an essential regulator of circadian rhythms. Clock is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for Clocks nuclear translocation, E box dependent transcription, phosphorylation, and degradation.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting: 0.5ug/mL
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.5ug/ml
Immunogen:
E. coli-derived recombinant human Clock.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 0.02% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.
Specificity:
Recognizes human Clock.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.