Technical Data
C5851-80H
Clostridium botulinum Toxin A (BoNT/A, Bontoxilysin-A)
Description:
Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln--Arg-198 bond of SNAP-25.

Applications:
Suitable for use in Dot Blot, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRabbit
Concentration:
~1.5mg/ml
Immunogen:
Type A Neurotoxin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 1X PBS, pH 7.2, 20% glycerol. No preservative added.
Specificity:
Recognizes Clostridium botulinum Type A Neurotoxin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.