Technical Data
Media
Clostridium Difficile Agar (Powder)
C5852
Microbiological Grade
Storage 4C    Shipping RT
Components shown as g/liter
Proteose Peptone40.0
Fructose6.0
Monopotassium Phosphate10.0
Disodium Phosphate5.0
Magnesium Sulfate0.1
Sodium Chloride2.0
Agar15.0
Total:78.1g/liter
For isolation of Clostridium difficile.
Clostridium Difficile Agar is a formula recommended by George et al. for isolation of C. difficile. The medium contains fructose which enhances the recovery and growth of C. difficile. The addition of the antibiotics, cefoxitin and cycloserine, inhibit the growth of Enterobacteriaceae, Enterococcus faecalis, staphylococci, Gram negative non-sporulating anaerobic bacilli, and Clostridia sp. but not C. difficile. Supplementing the medium with horse blood and egg yolk further improves the recovery of C. difficile.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 8.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 78.1grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). 3.Cool to 45-50C and add 7% of horse blood and Cycloserine (0.5 g/L) and Cefoxitin (0.016 g/L). Alternately, neutral red (0.03 g/L) may be added prior to autoclaving and Cycloserine and Cefoxitin added after autoclaving.Storage:Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Microbiological Analysis: Bacteroides fragilis ATCC 25285

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light amber,clear to slightly hazy.

pH (25C): 7.40.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.