Technical Data
Media
Clostrisel Agar (Powder)
C5855
Microbiological Grade
Storage 4C    Shipping RT
Components shown as g/liter
Agar14.0
L-Cystine0.25
Sodium Thioglycollate1.8
Dextrose6.0
Neomycin Sulfate0.15
Sodium Azide0.20
Sodium Chloride2.5
Sodium Formaldehyde Sulfoxylate 1.0
Papaic Digest of Soybeans3.0
Casein Digest Peptone16.0
Total:45g/liter
Highly selective medium for recovery of clostridia from clinical and other sources.

Clostrisel Agar was first described by Vera for the isolation of pathogenic Clostridia from mixed flora specimens (wounds, soil, feces and other contaminated material). The nutrient base for this formulation is Tryptic Soy Broth with the addition of reducing agents sodium thioglycollate and sodium formaldehyde sulfoxylate which provide a low oxidation-reduction potential necessary for the growth of obligate anaerobes. Inhibition of Bacillus, Enteric bacteria, Pseudomonas, Proteus and most Gram positive organisms (except Enterococcus) is achieved by the addition of neomycin and sodium azide.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Directions per Liter: Mix thoroughly 45 g of the powder with 1L of distilled water. Heat with frequent agitation and boil for 1 minute to dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Light amber,clear to turbid with heat.

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.