Technical Data
Media
CMRL1066 w/o L-Glutamine, Phenol Red (Powder)
C5900-03A
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride, anhydrous0.2
Magnesium Sulfate, anhydrous0.09769
Potassium Chloride0.4
Sodium Acetate, anhydrous0.05
Sodium Chloride6.8
Sodium Phosphate Monobasic, anhydrous0.122
Amino Acids:
L-Alanine0.025
L-Arginine0.05787
L-Aspartic Acid0.03
L-Cysteine•HCL•H2O0.26
L-Cystine•2HCl0.026
L-Glutamic Acid, Sodium0.075
L-GlutamineAbsent
Glycine0.05
L-Histidine•HCl•H2O0.02
trans-4-Hydroxy-L-Proline0.01
L-Isoleucine0.02
L-Leucine0.06
L-Lysine•HCl0.07
L-Methionine0.015
L-Phenylalanine0.025
L-Proline0.04
L-Serine0.025
L-Threonine0.03
L-Tryptophan0.01
L-Tyrosine0.04
L-Valine0.025
Vitamins:
PABA0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride0.0005
CoenzymeA•Na 0.0025
Cocarboxylase0.001
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl0.0116
2'-Deoxyguanosine0.01
FolicAcid0.00001
myo-Inositol0.00005
5-Methyldeoxycytidine0.0001
ß-NAD0.007
ß-NADP•Na0.001
NicotinicAcid0.000025
Niacinamide0.000025
D-PantothenicAcid•Ca0.00001
Pyridoxal•HCl0.000025
Pyridoxine•HCl0.000025
Riboflavin0.00001
Flavin Adenine Dinucleotide•2Na0.000106
Thiamine•HCl0.00001
Other:
Cholesterol0.0002
Thymidine0.01
D-Glucose1.00
D-GlucuronicAcid•Na0.00388
Glutathione0.01
Phenol Red, SodiumAbsent
Tween800.005
Uridine-5-Triphosphate•4Na0.001
Total9.7g/liter
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.


Directions per Liter: Dissolve 9.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add 2.2 grams sodium bicarbonate with stirring. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Mulay, V.B. et al., (2009) J. Bacteriology 191:2783-2794. 1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966). 2. Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O. 3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper and Row Publishers Inc., New York. Ed.: Parker, R.C.