Technical Data
Media
CMRL1066 10X w/o L-Glutamine (Powder)
C5900-05
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter (10X)
Inorganic Salts:
Calcium Chloride2.00
Magnesium Sulfate, anhy0.9769
Potassium Chloride4.00
Sodium Acetate, anhy0.50
Sodium Chloride68.0
Sodium Phosphate Mono, anhy1.22
Amino Acids:
L-Alanine0.25
L-Arginine0.5787
L-Aspartic Acid0.30
L-Cysteine•HCL•H2O2.60
L-Cystine0.20
L-Glutamic Acid, Sodium0.862
L-GlutamineAbsent
Glycine0.50
L-Histidine•HCl•H2O0.20
trans-4-Hydroxy-L-Proline0.10
L-Isoleucine0.20
L-Leucine0.60
L-Lysine•HCl0.70
L-Methionine0.15
L-Phenylalanine0.25
L-Proline0.40
L-Serine0.25
L-Threonine0.30
L-Tryptophan0.10
L-Tyrosine0.40
L-Valine0.25
Vitamins:
PABA0.0005
L-Ascorbic Acid•Na0.50
D-Biotin0.0001
Choline Chloride0.005
Coenzyme A•Na0.025
Cocarboxylase0.01
2'-Deoxyadenosine0.10
2'-Deoxycytidine•HCl0.116
2'-Deoxyguanosine0.10
Folic Acid0.0001
myo-Inositol0.0005
5-Methyldeoxycytidine0.001
ß-NAD0.070
ß-NADP•Na0.010
Nicotinic Acid0.00025
Niacinamide0.00025
D-Pantothenic Acid•Ca0.0001
Pyridoxal•HCl0.00025
Pyridoxine•HCl0.00025
Riboflavin0.0001
Flavin Adenine Dinucleotide•2Na0.00106
Thiamine•HCl0.0001
Other:
Cholesterol0.002
Thymidine0.10
D-Glucose10.0
D-Glucuronic Acid•Na0.0388
Glutathione0.10
Phenol Red, Sodium0.2124
Tween 800.05
Uridine-5-Triphosphate•4Na0.0113
Total:97.34g/Liter
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.

Note: USB recommends the preparation of this product as a 1X solution.



Directions per Liter of 10X: Dissolve 97.34g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 22g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Some precipitate may be observed due to excess concentration of salts. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Orange, homogeneous, free flowing powder

Solubility: Yellow to orange, clear, complete. Some precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.

pH: 3.4 ± 0.4

Endotoxin: 1EU/ml

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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Stewart, P.E. and Rosa, P.A. (2008) in Methods in Molecular Biology: Bacterial Pathogenesis 431: 85-95.
1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966). 2.Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O. 3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper and Row Publishers Inc., New York. Ed.: Parker, R.C.