Technical Data

Media

CMRL1066 w/L-Glutamic Acid, Proline, High Choline Chloride, D-Pantothenic Acid (Powder)

C5900-08

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as g/liter

Inorganic Salts:
Calcium Chloride, anhy	0.2
Magnesium Sulfate, anhy	0.09769
Potassium Chloride	0.4
Sodium Acetate, anhy	0.05
Sodium Chloride	6.8
Sodium Phosphate Monobasic, anhy	0.122

Amino Acids:
L-Alanine	Absent
L-Arginine	Absent
L-Aspartic Acid	Absent
L-Cysteine•HCL•H2O	Absent
L-Cystine•2HCl	Absent
L-Glutamic Acid, Sodium	0.075
L-Glutamine	Absent
Glycine	Absent
L-Histidine•HCl•H2O	Absent
trans-4-Hydroxy-L-Proline	Absent
L-Leucine	Absent
L-Lysine•HCl	Absent
L-Methionine	Absent
L-Phenylalanine	Absent
L-Proline	0.04
L-Serine	Absent
L-Threonine	Absent
L-Tryptophan	Absent
L-Tyrosine	Absent
L-Valine	Absent

Vitamins:
PABA	0.00005
L-Ascorbic Acid•Na	0.05
D-Biotin	0.00001
Choline Chloride	0.001
Coenzyme A•Na	0.0025
Cocarboxylase	0.001
2'-Deoxyadenosine	0.01
2'-Deoxycytidine•HCl	0.0116
2'-Deoxyguanosine	0.01
Folic Acid	0.00001
myo-Inositol	0.00005
5-Methyldeoxycytidine	0.0001
b-NAD	0.007
b-NADP•Na	0.001
Nicotinic Acid	0.000025
Niacinamide	0.000025
D-Pantothenic Acid•Ca	0.00004
Pyridoxal•HCl	0.000025
Pyridoxine•HCl	0.000025
Riboflavin	0.00001
Flavin Adenine Dinucleotide•2Na	0.000106
Thiamine•HCl	0.00001

Other:
Cholesterol	0.0002
Thymidine	0.01
D-Glucose	1.00
D-GlucuronicAcid•Na	0.00388
Glutathione	0.01
Phenol Red,Sodium	0.02124
Tween 80	0.005
Uridine-5-Triphosphate•4Na	0.001

Total	8.93g/liter

CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.

Directions per Liter: Dissolve 8.93g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Orange, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As Reported

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.