Technical Data
Media
CMRL1066 w/L-Glutamic Acid, Proline, High Choline Chloride, D-Pantothenic Acid (Powder)
C5900-08
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride, anhy0.2
Magnesium Sulfate, anhy0.09769
Potassium Chloride0.4
Sodium Acetate, anhy0.05
Sodium Chloride6.8
Sodium Phosphate Monobasic, anhy0.122
Amino Acids:
L-AlanineAbsent
L-ArginineAbsent
L-Aspartic AcidAbsent
L-Cysteine•HCL•H2OAbsent
L-Cystine•2HClAbsent
L-Glutamic Acid, Sodium0.075
L-GlutamineAbsent
GlycineAbsent
L-Histidine•HCl•H2OAbsent
trans-4-Hydroxy-L-ProlineAbsent
L-LeucineAbsent
L-Lysine•HClAbsent
L-MethionineAbsent
L-PhenylalanineAbsent
L-Proline0.04
L-SerineAbsent
L-ThreonineAbsent
L-TryptophanAbsent
L-TyrosineAbsent
L-ValineAbsent
Vitamins:
PABA0.00005
L-Ascorbic Acid•Na0.05
D-Biotin0.00001
Choline Chloride0.001
Coenzyme A•Na0.0025
Cocarboxylase0.001
2'-Deoxyadenosine0.01
2'-Deoxycytidine•HCl0.0116
2'-Deoxyguanosine0.01
Folic Acid0.00001
myo-Inositol0.00005
5-Methyldeoxycytidine0.0001
b-NAD0.007
b-NADP•Na0.001
Nicotinic Acid0.000025
Niacinamide0.000025
D-Pantothenic Acid•Ca0.00004
Pyridoxal•HCl0.000025
Pyridoxine•HCl0.000025
Riboflavin0.00001
Flavin Adenine Dinucleotide•2Na0.000106
Thiamine•HCl0.00001
Other:
Cholesterol0.0002
Thymidine0.01
D-Glucose1.00
D-GlucuronicAcid•Na0.00388
Glutathione0.01
Phenol Red,Sodium0.02124
Tween 800.005
Uridine-5-Triphosphate•4Na0.001
Total8.93g/liter
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.


Directions per Liter: Dissolve 8.93g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Orange, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.