Technical Data
Media
CMRL1066 w/L-Glutamine, w/o B-NAD (Powder)
C5900-09
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride, anhy 0.2
Magnesium Sulfate, anhy 0.09769
Potassium Chloride 0.4
Sodium Acetate, anhy 0.05
Sodium Chloride 6.8
Sodium Phosphate Monobasic, anhy 0.122
Amino Acids:
L-Alanine 0.025
L-Arginine0.05787
L-Aspartic Acid 0.03
L-Cysteine•HCL•H2O 0.26
L-Cystine•2HCl 0.02
L-Glutamic Acid, Sodium 0.075
L-Glutamine 0.1
Glycine 0.05
L-Histidine•HCl•H2O 0.02
trans-4-Hydroxy-L-Proline 0.01
L-Isoleucine 0.02
L-Leucine 0.06
L-Lysine•HCl 0.07
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.04
L-Serine 0.025
L-Threonine 0.03
L-Tryptophan 0.01
L-Tyrosine 0.04
L-Valine 0.025
Vitamins:
PABA 0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride 0.0005
Coenzyme A•Na 0.0025
Cocarboxylase 0.001
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl 0.0116
2'-Deoxyguanosine 0.01
Folic Acid 0.00001
myo-Inositol 0.00005
5-Methyldeoxycytidine 0.0001
b-NAD absent
b-NADP•Na 0.001
Nicotinic Acid 0.000025
Niacinamide 0.000025
D-Pantothenic Acid•Ca 0.00001
Pyridoxal•HCl 0.000025
Pyridoxine•HCl 0.000025
Riboflavin 0.00001
Flavin Adenine Dinucleotide•2Na 0.000106
Thiamine•HCl 0.00001
Other:
Cholesterol 0.0002
Thymidine 0.01
D-Glucose 1.00
D-Glucuronic Acid•Na 0.00388
Glutathione 0.01
Phenol Red,Sodium 0.02124
Tween 80 0.005
Uridine-5-Triphosphate•4Na 0.001
Total9.82g/L
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.


Directions per Liter: Dissolve 9.82g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Orange, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Healy, G.M., Parker, R.C.: An Improved Chemically Defined Basal Medium (CMRL-1415) For Newly Explanted Mouse Embryo Cells. Journal of Cell Biology 30: 531-538 (1966). 2.Parker, R.C., et al.: Altered Cell Strains In Continuous Culture: A General Survey In: Special Publications of the New York Academy of Sciences 5: 303-313 (1957). Publisher: N.Y. Acad. of Sci.. Ed.: Whitelock, O. 3. Methods of Tissue Culture, Third Edition, pp. 62-80. Publisher: Harper and Row Publishers Inc., New York. Ed.: Parker, R.C.