Technical Data
C6200-01F
CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase)
Description:
Murine CNPase (EC 3.1.4.37) is a 47.122 kD protein (420 aa) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells
Western Blot: 1:2000-1:5000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseChicken
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to a region of the CNPase gene product (KLH). Species sequence homology: human (NP_149124, NCBI) and mouse (P16330, NCBI) 100%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes CNPase. Species crossreactivity: mouse. Species sequence homology: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.