Technical Data
C6205-01
CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase)
Description:
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. CNPase has been very useful as a marker for these particular cell types. Developmentally, this enzyme appears early in oligodendrocytes, earlier than most other myelin proteins. It continues to be expressed at high levels in oligodendrocytes and Schwann cells of adult animals. In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNPase levels may be severely reduced. The enzyme itself exists in two forms in most species (three in mouse) with molecular masses of 48 and 46kD on SDS-PAGE, referred to as CNP2 and CNP1, respectively.Studies continue to elucidate the physiological role of the enzyme. CNPase I (46kD) and CNPase II (48kD) are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.

Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system. This protein is also recognized by the CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase) Mab Mo xHu (Catalog No: C6205-01). In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 10ug/ml
Immunohistochemistry: 10ug/ml
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q34Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human brain CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase) (whole protein).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Reacts with both human CNP1 and CNP2. Species Crossreactivity: Bsheep, canine, sheep, mouse, rabbit and rat. Bsheep, canine, human, mouse, porcine, rabbit, rat, sheep. Does not crossreact with Chicken, Guinea Pig. Reactivity with other species has not been confirmed. Recognizes humn CNPase, 48 and 46kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc. Species Crossreactivity: Bsheep, rat, mouse, rabbit, sheep, porcine, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sprinkle, T.J., (1989) CRC Crit. Rev. Neurobiol. 4(3): 235. 2. Kim, S.U., et al., (1984) Brain Res. 300: 195. 3. Sheedlo, H.J. and Sprinkle, T.J., (1983) Brain Res. 288: 330. 4. McMorris, F.A., et al., (1984) Brain Res. 292: 123. 5. Sprinkle, T.J., et al., (1987) Brain Res. 426: 349. 6. Sprinkle, T.J., et al., (1991) Soc. Neurosci. Abstr. 17.1: 376. 7. Sprinkle, T.J., et al., (1978) J. Neurochem. 30: 309. 8. Hsu, S.M., et al., (1981) Am. J. Clin. Pathol. 75: 734. 9. Falini, B. and Taylor, C.R., (1983) Arch. Pathol. Lab. Med. 107: 105. 10. Harlow, E. and Lane, D., (1988) Antibodies: A Laboratory Manual, p. 359, Cold Spring Harbor Laboratory, N.Y. 11. Taylor, C.R., (1978) Arch. Pathol. Lab. Med. 102: 113.