Technical Data
CNPY3 (Canopy 3 Homolog, Protein Canopy Homolog 3, TNRC5, Trinucleotide Repeat-containing Gene 5 Protein, Prat4a)
PRAT4A (Protein associated with TLR4-A) is a ubiquitously expressed, 40kD protein that participates in Toll receptor expression. Mouse PRAT4A is 276aa in length. It contains a putative signal sequence of 37aa, followed by a 239aa mature region. Although the presence of a signal sequence suggests it is secreted, its best known activity involves an intracellular association with TLR4. PRAT4A appears to facilitate the maturation and expression of TLR4 on the cell surface. Studies suggest this involves the C-terminal 60aa. Mature mouse PRAT4A shares 90% and 96% aa identity with human and rat PRAT4A, respectively.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceMouseGoat
As reported
Purified, NS0-derived, recombinant mouse CNPY3 isoform 1 (aa38-276; Accession #Q9DAU1).
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
Recognizes mouse CNPY3. Species sequence homology: human (90%), rat (96%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.