Technical Data
CNPY3 (PRAT4A, Protein Canopy Homolog 3, CTG Repeat Protein 4a, Expanded Repeat-domain Protein CAG/CTG 5, Protein Associated with TLR4, Trinucleotide Repeat-containing Gene 5 Protein, CTG4A, ERDA5, TNRC5, HSPC084, UNQ1934/PRO4409)
PRAT4A is a novel protein belonging to the canopy family with a saposin B-type domain. It is an accessory molecule required for microbial recognition by Toll-like receptor (TLR), and mediates TLR dependent immune responses. Though the exact function of PRAT4A is still unknown, reports suggest that it is associated with the immature form of TLR4 and regulates the cell surface expression of this TLR. PRAT4A, a secreted protein, also has a role in TLR4 maturation and glycosylation. It is usually present in endoplasmic reticulum and plays an active role in regulating the subcellular distribution and response of multiple TLRs and is required for both innate and adaptive immune responses.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa200-250 of human PRAT4A.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human PRAT4A. Species Crossreactivity: mouse and rat. Species sequence homology: equine, chimpanzee, pocine, canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wakabayashi, Y. et al. J. Immun. 177:1772-1779 (2006). 2. Takahashi, K. et al. J Exp Med. 204:2963-2976 (2007). 3. Akashi-Takamura, S. et al. Curr Opin Immunol. 20:420-425 (2008).