Technical Data
Cofilin is a small phosphoinositide-sensitive, actin-binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their

Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Detects a band at approximately 19kDa. Use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells, or purified recombinant chicken muscle cofilin.
Immunofluorescence: 1:1000 using NIH-NT3 fibroblasts
Optimal dilutions to be determined by the researcher.

Cellular Localization: Cytoplasmic and Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
50ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin
Supplied as a liquid in PBS, pH 7.4, containing sodium azide
Recognizes human Cofilin. Species Crossreactivity: mouse, rat, chicken, canine and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nishida, E., et al., Biochemistry, 23, 5307-5316 (1984).
2. Nishida, E., et al., Proc. Natl. Acad. Sci. (USA), 84, 5262-5266 (1987).
3. Moriyama, K., and Yahara, I., EMBO J., 18, 6752-6761 (1999).
4. Bamburg, R., Annu. Rev. Cell. Dev. Biol., 15, 185-230 (1999).
5. Carlier, M.F., et al., J. Cell Biol., 136, 1307-1322 (1997).
6. Maciver, S.K., et al., J. Cell. Biol., 278, 1611-1620 (1991).
7. Ohta, Y., et al., J. Biol. Chem., 264, 16143-16148 (1989).
8. Suzuki, K.T., et al., J. Biol. Chem., 270, 19551-19556 (1997).
9. Minamide, A., et al., Nature Cell Biol., 2, 628-636 (2000).
10. Arber, S., et al., Nature, 393, 805-809 (1998).