Technical Data
C7506-25E
Cofilin, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin, Non-muscle Isoform, CFL1, CFL)
Description:
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic phospho-peptide corresponding to the phosphorylation site of serine 3 (M-A-SP-G-V) from human Cofilin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of human Cofilin only when phosphorylated at serine 3. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kobayashi M., et al. (2006) EMBO J 25(4):713-26. 2. Wany Y, et al. (2005) Biol Chem 280(13):12683-9. 3. Smith-Beckerman DM., et al. (2005) Mol Cell Proteomics 156-68.