Technical Data
C7510-17B
Collagen Type I
Description:
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600 in length and 15 in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Applications:
Suitable for use in Immunofluorescence, ELISA, RIA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: >1:80 on frozen rat tissues
Immunohistochemistry (paraffin): >1:500
ELISA: >1:200
RIA (rat): 1: 200
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Collagen Type I puri ed from rat skin.
Purity:
Purified by ion exchange chromatography (DEAE-Trisacryl).
Form
Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes rat collagen Type I. Does not crossreact with rat collagen Type III and rat elastin. Does not crossreact with collagen Type I from human and chicken sources. Crossreacts with mouse collagen Type I when tested by ELISA and Immunohistochemistry but does not crossreact when tested by RIA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.