Technical Data
C7510-17J
Collagen Type I
Description:
Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.

Applications:
Suitable for use in Immunohistochemistry, RIA, Western Bot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:40 for immunofluorescent staining of frozen mouse skin and liver tissues
RIA: 1:200
Western Blot: 1:500. Detects Collegen Type 1 on 10ug of mouse liver lysate
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Collagen Type 1 extracted and purified from mouse skin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added.
Specificity:
Recognizes mouse Collagen Type 1. <0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagen Type I. 1% reactivity with mouse Collagen Type III.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ramshaw, J A and Werkmeister, J A (1988) Anal. Biochem., 168: 82-7 (1988) 2. Martínez-Salgado, Carlos, et al (2006) Exp Cell Res, 312:2093-106 (2006) 3. Phillips, Roderick J, et al. (2004) J. Clin. Invest., 114: 438-46 (2004)