Technical Data
Collagen Type I
Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals, and it is also widespread all over the body. In addition to bones, it is found in skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues among other locations. Even cartilage, which mainly contains type II collagen, contains of some type I collagen. It is also widespread in the animal kingdom, from invertebrates to vertebrates, and moreover it is restricted to animals; plants do not have true collagens. Type I collagen is also important in other respects. For example, it is a large component of gelatin (JelloTM) and in many biomaterials, indeed, leather is mostly composed of type I collagen. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Because of of its wide distribution thoughtout the body, the monitoring of the breakdown of type I collagen molecule is fequently used to monitor the physiological changes in tissues and can be used as a diagnostic tool in various pathological conditions.

Suitable for use in Immunohistochemistry, Immunofluorescence, RIA, ELISA. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:80 for immunofluorescent staining of frozen bovine liver and skin tissues. Formalin fixed, paraffin embedded tissues requires HIER antigen retrieval and enhanced enzymatic detection. Methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
ELISA (direct): 1:400
RIA: 1:500
Optimal dilution to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceBovineRabbit
Purified bovine type I collagen extracted from purified bovine skin.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2. No preservatives.
Recognizes bovine collagen type I. Crossreactivity: <0.1% with bovine collagen types II, IV, XI or bovine fibrogen by RIA, <2% with human collagen type I by RIA, and <0.1% with chicken collagen type I by RIA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.