Technical Data
C7510-17V
Collagen Type I (Biotin)
Description:
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600 in length and 15 in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Applications:
Suitable for use in ELISA, Dot and Slot Blots, Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Western Blots. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:4000
Dot/Slot Blot: 1:500-1:1000
Western Blot: reduced only
Indirect Immunohistochemistry (Frozen sections only): 1:20-1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanGoat
Concentration:
~0.1mg/ml
Immunogen:
Human placental collagen type I
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Biotin.
Specificity:
Recognizes human Collagen Type I. Exhibits <10% crossreactivity with collagen types II, III, IV, V and VI. May crossreact with Collagen Type I from other species. Not tested for crossreactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.